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In situ quantification và evaluation of ClO−/H2S homeostasis in inflammatory gastric tissue by applying a rationally designed dual-response fluorescence probe featuring a novel H+-activated mechanism†


QiangFu,‡ab GuangChen, ‡*ac YuxiaLiu,a ZipingCao,a XianenZhao,a GuoliangLi,a FabiaoYu,c LingxinChen, c HuaWang *aand JinmaoYou*ab

* Corresponding authors

a The Key Laboratory of Life-Organic Analysis, Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University, Qufu 273165, Shandong, china E-mail: chenandguang


Xem thêm: Anh Hỏi Em Rằng Sau Này Chúng Mình Nếu Được Thành Đôi, Mộng Ước Đôi Ta

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b Qinghai Normal University, Xining, đài loan trung quốc

c Key Laboratory of Coastal Environmental Processes và Ecological Remediation, The Research Centre for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, trung quốc


Homeostasis of ClO−/H2S plays a crucial role in the damage & repair of gastric tissue, but has rarely been investigated due khổng lồ the challenge of in situ analysis in the highly acidic gastric environment. Herein, we designed a new H+-activated optical mechanism, involving controllable photoinduced electron transfer (PET) & switch of electron push–pull (SEPP), to lớn develop the simple yet multifunctional probe (Z)-4-(2-benzylidenehydrazinyl)-7-nitrobenzo<c><1,2,5>oxadiazole (BNBD). First, the BNBD probe (Off) was protonated by the highly acidic truyền thông to trigger strong fluorescence (On). Then, the analytes ClO− & H2S reacted with the protonated BNBD, leading lớn ultrasensitive (ClO−: 2.7 nM and H2S: 6.9 nM) fluorescence quenching via the rapid oxidation of C

*
" />N (50 s) & nitro reduction (10 s), respectively. With the logical discrimination by absorbance/colour (ClO−: 300 nm/colorless and H2S: 400 nm/orange), a strategy for the in situ quantification of ClO−/H2S in gastric mucosa & juice was developed. For the first time, the in situ quantitative monitoring of endogenous H2S & ClO−/H2S homeostasis as well as the pathologic manifestation in gastric mucosa were realized, thus overcoming the challenge of ClO−/H2S analysis under highly acidic conditions và enabling the in situ tissue quantification of ClO−/H2S. In combination with the assessment of mucosal damage, this study confirms the injurious/rehabilitative effects of ClO−/H2S on gastric mucosa (at 50–90 μm depth), which may facilitate the auxiliary diagnosis of stomach diseases induced by oxidative stress.